Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 647126..647745 Replicon chromosome
Accession NZ_CP063954
Organism Klebsiella variicola strain S129-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag IOD50_RS03080 Protein ID WP_002892050.1
Coordinates 647126..647344 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag IOD50_RS03085 Protein ID WP_008805436.1
Coordinates 647371..647745 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD50_RS03045 643169..643432 + 264 WP_008805431.1 type B 50S ribosomal protein L31 -
IOD50_RS03050 643432..643572 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
IOD50_RS03055 643569..644267 - 699 WP_016160391.1 GNAT family N-acetyltransferase -
IOD50_RS03060 644368..645825 + 1458 WP_172749140.1 PLP-dependent aminotransferase family protein -
IOD50_RS03065 645794..646264 - 471 WP_008805434.1 YlaC family protein -
IOD50_RS03070 646301..646429 + 129 Protein_597 hypothetical protein -
IOD50_RS03075 646401..646967 - 567 WP_008805435.1 maltose O-acetyltransferase -
IOD50_RS03080 647126..647344 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
IOD50_RS03085 647371..647745 - 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
IOD50_RS03090 648228..651374 - 3147 WP_064179935.1 multidrug efflux RND transporter permease subunit AcrB -
IOD50_RS03095 651397..652590 - 1194 WP_043875731.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T179828 WP_002892050.1 NZ_CP063954:c647344-647126 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T179828 NZ_CP084895:4458223-4458573 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT179828 WP_008805436.1 NZ_CP063954:c647745-647371 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT179828 NZ_CP084895:4457978-4458229 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References