Detailed information of TA system TA179827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4197366..4198954
• Replicon: chromosome
• Accession: NZ_CP063953
• Organism: Klebsiella variicola strain S131-2

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: ISD12_RS20040
• Protein ID: WP_194394605.1
• Coordinates: 4197366..4198688 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A1F2MCN9
• Locus tag: ISD12_RS20045
• Protein ID: WP_008804920.1
• Coordinates: 4198688..4198954 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISD12_RS20025	4192431..4194932	+	2502	WP_194394603.1	fimbrial biogenesis outer membrane usher protein	-
ISD12_RS20030	4194920..4195924	+	1005	WP_194394604.1	type 1 fimbrial protein	-
ISD12_RS20035	4196057..4197349	+	1293	WP_012968243.1	glycoside hydrolase family 10 protein	-
ISD12_RS20040	4197366..4198688	-	1323	WP_194394605.1	type II toxin-antitoxin system HipA family toxin	Toxin
ISD12_RS20045	4198688..4198954	-	267	WP_008804920.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
ISD12_RS20050	4199220..4199546	-	327	WP_008804921.1	hydroxyisourate hydrolase	-
ISD12_RS20055	4199543..4200043	-	501	WP_008804922.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
ISD12_RS20060	4200021..4201400	-	1380	WP_012968246.1	purine permease	-
ISD12_RS20065	4201746..4202900	+	1155	WP_023322603.1	FAD-dependent urate hydroxylase HpxO	-
ISD12_RS20070	4202881..4203804	-	924	WP_008804925.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 48795.14 Da
• Isoelectric Point: 7.9109

>T179827 WP_194394605.1 NZ_CP063953:c4198688-4197366 [Klebsiella variicola]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGGHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMAFLLGSSEALKDRADFMKFMVFQWLVGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPGEQMLAILQEFAGHVP
QAIASARRTLPADFSAHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T179827 NZ_CP084895:4362439-4362612 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.30 Da
• Isoelectric Point: 8.5912

>AT179827 WP_008804920.1 NZ_CP063953:c4198954-4198688 [Klebsiella variicola]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKATETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT179827 NZ_CP084895:4362115-4362411 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A1F2MCN9