Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3270420..3271010 Replicon chromosome
Accession NZ_CP063953
Organism Klebsiella variicola strain S131-2

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag ISD12_RS15450 Protein ID WP_008804165.1
Coordinates 3270678..3271010 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag ISD12_RS15445 Protein ID WP_012541132.1
Coordinates 3270420..3270677 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISD12_RS15425 3266032..3266607 + 576 WP_016161434.1 hypothetical protein -
ISD12_RS15430 3266784..3267620 + 837 WP_008804155.1 alpha/beta hydrolase -
ISD12_RS15435 3267826..3268797 + 972 WP_008804156.1 sensor domain-containing diguanylate cyclase -
ISD12_RS15440 3268794..3269894 - 1101 WP_080928497.1 phosphotransferase -
ISD12_RS15445 3270420..3270677 + 258 WP_012541132.1 antitoxin Antitoxin
ISD12_RS15450 3270678..3271010 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ISD12_RS15460 3271333..3272769 + 1437 WP_042948427.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
ISD12_RS15470 3273142..3274596 - 1455 WP_064160537.1 AMP nucleosidase -
ISD12_RS15475 3274725..3274970 - 246 WP_008804171.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T179818 WP_008804165.1 NZ_CP063953:3270678-3271010 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T179818 NZ_CP084895:c2094681-2094430 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT179818 WP_012541132.1 NZ_CP063953:3270420-3270677 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT179818 NZ_CP084895:c2094433-2094179 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References