Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2249899..2250556 Replicon chromosome
Accession NZ_CP063953
Organism Klebsiella variicola strain S131-2

Toxin (Protein)


Gene name cptA Uniprot ID W8UCT0
Locus tag ISD12_RS10680 Protein ID WP_002916310.1
Coordinates 2250146..2250556 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag ISD12_RS10675 Protein ID WP_002916312.1
Coordinates 2249899..2250165 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISD12_RS10650 2244996..2246429 - 1434 WP_063105732.1 6-phospho-beta-glucosidase BglA -
ISD12_RS10655 2246549..2247277 - 729 WP_012967245.1 MurR/RpiR family transcriptional regulator -
ISD12_RS10660 2247328..2247639 + 312 WP_008806430.1 N(4)-acetylcytidine aminohydrolase -
ISD12_RS10665 2247803..2248462 + 660 WP_008806429.1 hemolysin III family protein -
ISD12_RS10670 2248670..2249653 - 984 WP_080930388.1 tRNA-modifying protein YgfZ -
ISD12_RS10675 2249899..2250165 + 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
ISD12_RS10680 2250146..2250556 + 411 WP_002916310.1 protein YgfX Toxin
ISD12_RS10685 2250563..2251084 - 522 WP_008806427.1 flavodoxin FldB -
ISD12_RS10690 2251185..2252081 + 897 WP_110204188.1 site-specific tyrosine recombinase XerD -
ISD12_RS10695 2252104..2252817 + 714 WP_061153358.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ISD12_RS10700 2252823..2254556 + 1734 WP_049156820.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16049.85 Da        Isoelectric Point: 11.4778

>T179817 WP_002916310.1 NZ_CP063953:2250146-2250556 [Klebsiella variicola]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 411 bp

>T179817 NZ_CP084895:c2094433-2094179 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT179817 WP_002916312.1 NZ_CP063953:2249899-2250165 [Klebsiella variicola]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT179817 NZ_CP084895:c2094681-2094430 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GSW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References