Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1754064..1754695 Replicon chromosome
Accession NZ_CP063953
Organism Klebsiella variicola strain S131-2

Toxin (Protein)


Gene name hicA Uniprot ID A0A2V3KJT3
Locus tag ISD12_RS08100 Protein ID WP_044650325.1
Coordinates 1754064..1754339 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R4YIG2
Locus tag ISD12_RS08105 Protein ID WP_004181489.1
Coordinates 1754336..1754695 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISD12_RS08080 1749300..1749635 + 336 WP_002921203.1 universal stress protein UspB -
ISD12_RS08085 1749695..1751191 - 1497 WP_008806938.1 inorganic phosphate transporter PitA -
ISD12_RS08090 1751421..1752614 + 1194 WP_012967106.1 NAD(P)/FAD-dependent oxidoreductase -
ISD12_RS08095 1752654..1753667 - 1014 WP_012540371.1 magnesium transporter -
ISD12_RS08100 1754064..1754339 + 276 WP_044650325.1 type II toxin-antitoxin system HicA family toxin Toxin
ISD12_RS08105 1754336..1754695 + 360 WP_004181489.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ISD12_RS08110 1754723..1755124 - 402 WP_194394896.1 nickel-responsive transcriptional regulator NikR -
ISD12_RS08115 1755112..1755903 - 792 WP_012540373.1 nickel import ATP-binding protein NikE -
ISD12_RS08120 1755900..1756664 - 765 WP_023340796.1 nickel import ATP-binding protein NikD -
ISD12_RS08125 1756664..1757497 - 834 WP_194394897.1 nickel ABC transporter permease subunit NikC -
ISD12_RS08130 1757494..1758438 - 945 WP_008806948.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10279.91 Da        Isoelectric Point: 10.3165

>T179814 WP_044650325.1 NZ_CP063953:1754064-1754339 [Klebsiella variicola]
MEQQVLSLRNKQRHTLEQLFKIPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

>T179814 NZ_CP084895:c1648978-1648691 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13313.02 Da        Isoelectric Point: 4.4605

>AT179814 WP_004181489.1 NZ_CP063953:1754336-1754695 [Klebsiella variicola]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp

>AT179814 NZ_CP084895:c1649217-1648978 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V3KJT3

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M5Q1

References