Detailed information of TA system TA179812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 1077638..1079319
• Replicon: chromosome
• Accession: NZ_CP063953
• Organism: Klebsiella variicola strain S131-2

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: ISD12_RS05015
• Protein ID: WP_194394842.1
• Coordinates: 1077638..1078555 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: ISD12_RS05020
• Protein ID: WP_117107357.1
• Coordinates: 1078561..1079319 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISD12_RS04995	1072944..1074464	+	1521	WP_194394841.1	sugar ABC transporter ATP-binding protein	-
ISD12_RS05000	1074461..1075459	+	999	WP_023296904.1	ribose ABC transporter permease	-
ISD12_RS05005	1075490..1076431	+	942	WP_064144599.1	ABC transporter substrate-binding protein	-
ISD12_RS05010	1076506..1077513	+	1008	WP_023339423.1	carbohydrate kinase family protein	-
ISD12_RS05015	1077638..1078555	-	918	WP_194394842.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
ISD12_RS05020	1078561..1079319	-	759	WP_117107357.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
ISD12_RS05025	1079404..1080132	-	729	WP_008807258.1	response regulator transcription factor	-
ISD12_RS05030	1080405..1082552	+	2148	WP_012543160.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
ISD12_RS05035	1083170..1083856	+	687	WP_008807260.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-246)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34703.08 Da
• Isoelectric Point: 5.4707

>T179812 WP_194394842.1 NZ_CP063953:c1078555-1077638 [Klebsiella variicola]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGINAVLQANRSDEMRVVVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELNRPIFNGFIAYLLSHNRPLAEVLSPRWKDIAEPFYREFSGMTFETIALEELTAVPYRMIDALKS
CFTQQDIDFLLSFKRGEPDWRLAPETRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T179812 NZ_CP084895:1511610-1511786 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28683.04 Da
• Isoelectric Point: 10.2152

>AT179812 WP_117107357.1 NZ_CP063953:c1079319-1078561 [Klebsiella variicola]
MSSKLNWLLQNSAPGDVILQSWLSRHAISPSLAFKYTQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSMENKALLPKWFREFPGVEWIVISGQKLPVLDDRYRVLLDVKGKRLTGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQTKRLYLFFASFYEHGWLRRIDSQKIDLGAGKRQIVVNGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT179812 NZ_CP084895:1511832-1512248 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA