Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 394515..395155 Replicon plasmid pS166-1.1
Accession NZ_CP063946
Organism Klebsiella pneumoniae strain S166-1

Toxin (Protein)


Gene name vagD Uniprot ID -
Locus tag IOD25_RS27835 Protein ID WP_194433521.1
Coordinates 394515..394928 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag IOD25_RS27840 Protein ID WP_001261282.1
Coordinates 394925..395155 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD25_RS27815 389885..390235 + 351 WP_004187110.1 DUF305 domain-containing protein -
IOD25_RS27820 390421..391334 - 914 Protein_409 HNH endonuclease -
IOD25_RS27825 391872..392894 - 1023 WP_000361404.1 helicase UvrD -
IOD25_RS27830 392879..394441 - 1563 WP_004206609.1 AAA family ATPase -
IOD25_RS27835 394515..394928 - 414 WP_194433521.1 PIN domain-containing protein Toxin
IOD25_RS27840 394925..395155 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IOD25_RS27845 395112..395573 + 462 WP_074422511.1 hypothetical protein -
IOD25_RS27850 395734..396676 + 943 Protein_415 hypothetical protein -
IOD25_RS27855 396713..397105 + 393 WP_011977811.1 hypothetical protein -
IOD25_RS27860 397163..397684 + 522 WP_011977812.1 hypothetical protein -
IOD25_RS27865 397730..397933 + 204 WP_011977813.1 hypothetical protein -
IOD25_RS27870 397963..398967 + 1005 WP_011977814.1 hypothetical protein -
IOD25_RS27875 399153..399929 + 777 WP_194433541.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / qnrB4 / blaDHA-1 / mph(A) / aph(3')-Ia / sul2 / floR / tet(A) / blaLAP-2 / qnrS1 / dfrA14 / tet(D) - 1..433968 433968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-128)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 14920.31 Da        Isoelectric Point: 7.1084

>T179809 WP_194433521.1 NZ_CP063946:c394928-394515 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPDRAA
VDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 414 bp

>T179809 NZ_CP084895:c1270551-1270444 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT179809 WP_001261282.1 NZ_CP063946:c395155-394925 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT179809 NZ_CP084895:1270598-1270664 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References