Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 118500..119410 Replicon plasmid pS166-1.1
Accession NZ_CP063946
Organism Klebsiella pneumoniae strain S166-1

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag IOD25_RS26430 Protein ID WP_004026354.1
Coordinates 118940..119410 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag IOD25_RS26425 Protein ID WP_004181895.1
Coordinates 118500..118943 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD25_RS26390 113787..113966 - 180 WP_007372130.1 hypothetical protein -
IOD25_RS26395 114045..114722 + 678 Protein_124 IS1 family transposase -
IOD25_RS26400 115182..115382 + 201 Protein_125 transposase family protein -
IOD25_RS26405 115439..115764 - 326 Protein_126 transposase -
IOD25_RS26410 115852..116874 + 1023 WP_129304158.1 IS21-like element ISSen3 family transposase -
IOD25_RS26415 116871..117653 + 783 WP_032437422.1 IS21-like element ISSen3 family helper ATPase IstB -
IOD25_RS26420 117722..118399 + 678 Protein_129 DUF4113 domain-containing protein -
IOD25_RS26425 118500..118943 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
IOD25_RS26430 118940..119410 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
IOD25_RS26435 119520..119780 - 261 WP_004026352.1 hypothetical protein -
IOD25_RS26440 120475..120846 + 372 WP_194433529.1 helix-turn-helix domain-containing protein -
IOD25_RS26445 121016..121984 - 969 WP_194433530.1 IS5-like element IS903B family transposase -
IOD25_RS26450 122004..122906 + 903 WP_194433539.1 IS3 family transposase -
IOD25_RS26455 122915..123613 - 699 WP_040150695.1 arsenical resistance protein ArsH -
IOD25_RS26460 123699..124019 + 321 WP_040150694.1 transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / qnrB4 / blaDHA-1 / mph(A) / aph(3')-Ia / sul2 / floR / tet(A) / blaLAP-2 / qnrS1 / dfrA14 / tet(D) - 1..433968 433968
- inside IScluster/Tn - - 112223..131100 18877


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T179807 WP_004026354.1 NZ_CP063946:118940-119410 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T179807 NZ_CP084895:c479532-479314 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT179807 WP_004181895.1 NZ_CP063946:118500-118943 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT179807 NZ_CP084895:c479932-479558 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References