Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 5010871..5011646 Replicon chromosome
Accession NZ_CP063945
Organism Klebsiella pneumoniae strain S166-1

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A332L402
Locus tag IOD25_RS24540 Protein ID WP_021314147.1
Coordinates 5011161..5011646 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag IOD25_RS24535 Protein ID WP_004150912.1
Coordinates 5010871..5011164 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD25_RS24515 5006079..5006681 - 603 WP_004174410.1 short chain dehydrogenase -
IOD25_RS24520 5006779..5007690 + 912 WP_021314148.1 LysR family transcriptional regulator -
IOD25_RS24525 5007691..5008839 - 1149 WP_101999528.1 PLP-dependent aspartate aminotransferase family protein -
IOD25_RS24530 5008850..5010226 - 1377 WP_004174417.1 pyridoxal-phosphate dependent enzyme -
IOD25_RS24535 5010871..5011164 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
IOD25_RS24540 5011161..5011646 + 486 WP_021314147.1 GNAT family N-acetyltransferase Toxin
IOD25_RS24545 5012516..5013214 - 699 Protein_4789 IS1 family transposase -
IOD25_RS24550 5013269..5013730 + 462 Protein_4790 hypothetical protein -
IOD25_RS24555 5013827..5014043 + 217 Protein_4791 transposase -
IOD25_RS24565 5014558..5015271 - 714 WP_194433163.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17621.68 Da        Isoelectric Point: 8.5144

>T179804 WP_021314147.1 NZ_CP063945:5011161-5011646 [Klebsiella pneumoniae]
MILAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T179804 NZ_CP084895:c246239-245961 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT179804 WP_004150912.1 NZ_CP063945:5010871-5011164 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT179804 NZ_CP084895:c246502-246242 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332L402

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References