Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4194980..4195605 Replicon chromosome
Accession NZ_CP063945
Organism Klebsiella pneumoniae strain S166-1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag IOD25_RS20490 Protein ID WP_002882817.1
Coordinates 4194980..4195363 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IOD25_RS20495 Protein ID WP_004150355.1
Coordinates 4195363..4195605 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD25_RS20475 4192346..4193248 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IOD25_RS20480 4193245..4193880 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IOD25_RS20485 4193877..4194806 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IOD25_RS20490 4194980..4195363 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
IOD25_RS20495 4195363..4195605 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IOD25_RS20500 4195810..4196727 + 918 WP_194433093.1 alpha/beta hydrolase -
IOD25_RS20505 4196741..4197682 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IOD25_RS20510 4197727..4198164 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IOD25_RS20515 4198161..4199021 - 861 WP_023280688.1 virulence factor BrkB family protein -
IOD25_RS20520 4199015..4199614 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T179800 WP_002882817.1 NZ_CP063945:c4195363-4194980 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T179800 NZ_CP084894:4362439-4362612 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT179800 WP_004150355.1 NZ_CP063945:c4195605-4195363 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT179800 NZ_CP084894:4362115-4362411 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References