Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3968..4493 Replicon plasmid pBS329-2
Accession NZ_CP063944
Organism Klebsiella pneumoniae strain BS329-2

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag ISA55_RS25450 Protein ID WP_004197633.1
Coordinates 3968..4273 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag ISA55_RS25455 Protein ID WP_006788213.1
Coordinates 4275..4493 (-) Length 73 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA55_RS25420 502..615 + 114 WP_014343462.1 hypothetical protein -
ISA55_RS25425 744..1001 + 258 WP_194429505.1 hypothetical protein -
ISA55_RS25430 1059..1838 - 780 WP_194429504.1 site-specific integrase -
ISA55_RS25435 2036..3052 - 1017 WP_194429503.1 hypothetical protein -
ISA55_RS25440 3049..3372 - 324 WP_004197641.1 hypothetical protein -
ISA55_RS25445 3399..3800 - 402 WP_072199347.1 hypothetical protein -
ISA55_RS25450 3968..4273 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
ISA55_RS25455 4275..4493 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ISA55_RS25460 5090..5320 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
ISA55_RS25465 5317..5733 + 417 WP_186474563.1 type II toxin-antitoxin system VapC family toxin -
ISA55_RS25470 5774..6652 - 879 WP_064182004.1 restriction endonuclease -
ISA55_RS25475 7313..8590 + 1278 Protein_11 HlyD family secretion protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) - 1..104835 104835


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-100)

Antitoxin

(2-72)


Sequences


Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T179785 WP_004197633.1 NZ_CP063944:c4273-3968 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T179785 NZ_CP084894:1511610-1511786 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT179785 WP_006788213.1 NZ_CP063944:c4493-4275 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT179785 NZ_CP084894:1511832-1512248 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5


Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References