Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
Location | 3968..4493 | Replicon | plasmid pBS329-2 |
Accession | NZ_CP063944 | ||
Organism | Klebsiella pneumoniae strain BS329-2 |
Toxin (Protein)
Gene name | ccdB | Uniprot ID | J5VBT8 |
Locus tag | ISA55_RS25450 | Protein ID | WP_004197633.1 |
Coordinates | 3968..4273 (-) | Length | 102 a.a. |
Antitoxin (Protein)
Gene name | ccdA | Uniprot ID | A0A1D8K3R4 |
Locus tag | ISA55_RS25455 | Protein ID | WP_006788213.1 |
Coordinates | 4275..4493 (-) | Length | 73 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
ISA55_RS25420 | 502..615 | + | 114 | WP_014343462.1 | hypothetical protein | - |
ISA55_RS25425 | 744..1001 | + | 258 | WP_194429505.1 | hypothetical protein | - |
ISA55_RS25430 | 1059..1838 | - | 780 | WP_194429504.1 | site-specific integrase | - |
ISA55_RS25435 | 2036..3052 | - | 1017 | WP_194429503.1 | hypothetical protein | - |
ISA55_RS25440 | 3049..3372 | - | 324 | WP_004197641.1 | hypothetical protein | - |
ISA55_RS25445 | 3399..3800 | - | 402 | WP_072199347.1 | hypothetical protein | - |
ISA55_RS25450 | 3968..4273 | - | 306 | WP_004197633.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
ISA55_RS25455 | 4275..4493 | - | 219 | WP_006788213.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
ISA55_RS25460 | 5090..5320 | + | 231 | WP_001261276.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | - |
ISA55_RS25465 | 5317..5733 | + | 417 | WP_186474563.1 | type II toxin-antitoxin system VapC family toxin | - |
ISA55_RS25470 | 5774..6652 | - | 879 | WP_064182004.1 | restriction endonuclease | - |
ISA55_RS25475 | 7313..8590 | + | 1278 | Protein_11 | HlyD family secretion protein | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Conjugative plasmid | tet(A) | - | 1..104835 | 104835 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11585.27 Da Isoelectric Point: 6.4661
>T179785 WP_004197633.1 NZ_CP063944:c4273-3968 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T179785 NZ_CP084894:1511610-1511786 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8362.31 Da Isoelectric Point: 4.7405
>AT179785 WP_006788213.1 NZ_CP063944:c4493-4275 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW
Download Length: 219 bp
>AT179785 NZ_CP084894:1511832-1512248 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A2A5MCG5 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A1D8K3R4 |