Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4715308..4715933 Replicon chromosome
Accession NZ_CP063943
Organism Klebsiella pneumoniae strain BS329-2

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag ISA55_RS22865 Protein ID WP_002882817.1
Coordinates 4715308..4715691 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag ISA55_RS22870 Protein ID WP_004150355.1
Coordinates 4715691..4715933 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA55_RS22850 4712674..4713576 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
ISA55_RS22855 4713573..4714208 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
ISA55_RS22860 4714205..4715134 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
ISA55_RS22865 4715308..4715691 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
ISA55_RS22870 4715691..4715933 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
ISA55_RS22875 4716138..4717055 + 918 WP_012737090.1 alpha/beta hydrolase -
ISA55_RS22880 4717070..4718011 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
ISA55_RS22885 4718056..4718493 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
ISA55_RS22890 4718490..4719350 - 861 WP_004146232.1 virulence factor BrkB family protein -
ISA55_RS22895 4719344..4719943 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T179782 WP_002882817.1 NZ_CP063943:c4715691-4715308 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T179782 NZ_CP084894:c1270551-1270444 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT179782 WP_004150355.1 NZ_CP063943:c4715933-4715691 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT179782 NZ_CP084894:1270598-1270664 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References