Detailed information of TA system TA179781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4397461..4399142
• Replicon: chromosome
• Accession: NZ_CP063943
• Organism: Klebsiella pneumoniae strain BS329-2

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A6TH06
• Locus tag: ISA55_RS21395
• Protein ID: WP_015959269.1
• Coordinates: 4397461..4398336 (-)
• Length: 292 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: ISA55_RS21400
• Protein ID: WP_004177809.1
• Coordinates: 4398384..4399142 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA55_RS21375	4392767..4394287	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
ISA55_RS21380	4394284..4395282	+	999	WP_002885172.1	ribose ABC transporter permease	-
ISA55_RS21385	4395313..4396254	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
ISA55_RS21390	4396329..4397336	+	1008	WP_015959270.1	carbohydrate kinase family protein	-
ISA55_RS21395	4397461..4398336	-	876	WP_015959269.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
ISA55_RS21400	4398384..4399142	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
ISA55_RS21405	4399228..4399956	-	729	WP_002885152.1	response regulator transcription factor	-
ISA55_RS21410	4400229..4402376	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
ISA55_RS21415	4402606..4403292	+	687	WP_032413196.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (7-264)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 292 a.a.
• Molecular weight: 32990.17 Da
• Isoelectric Point: 5.4637

>T179781 WP_015959269.1 NZ_CP063943:c4398336-4397461 [Klebsiella pneumoniae]
MQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQAGMSAVLQANRSDE
MRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQHPRDFYDVKLLLDAQ
ELDRPIFNGFIVYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKSCITQQDVDFLLSFK
RGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 876 bp

>T179781 NZ_CP084894:c1270016-1269909 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT179781 WP_004177809.1 NZ_CP063943:c4399142-4398384 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT179781 NZ_CP084894:1270064-1270130 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Structures

Toxin

• AlphaFold DB: A6TH06

Antitoxin

• AlphaFold DB: A6TH05