Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3529917..3530536 Replicon chromosome
Accession NZ_CP063943
Organism Klebsiella pneumoniae strain BS329-2

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag ISA55_RS17210 Protein ID WP_002892050.1
Coordinates 3530318..3530536 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag ISA55_RS17205 Protein ID WP_002892066.1
Coordinates 3529917..3530291 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA55_RS17195 3525069..3526262 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
ISA55_RS17200 3526285..3529431 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
ISA55_RS17205 3529917..3530291 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
ISA55_RS17210 3530318..3530536 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
ISA55_RS17215 3530695..3531261 + 567 WP_002892030.1 maltose O-acetyltransferase -
ISA55_RS17220 3531233..3531373 - 141 WP_004147370.1 hypothetical protein -
ISA55_RS17225 3531394..3531864 + 471 WP_002892026.1 YlaC family protein -
ISA55_RS17230 3531839..3533290 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
ISA55_RS17235 3533391..3534089 + 699 WP_004177238.1 GNAT family N-acetyltransferase -
ISA55_RS17240 3534086..3534226 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
ISA55_RS17245 3534226..3534489 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T179777 WP_002892050.1 NZ_CP063943:3530318-3530536 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T179777 NZ_CP084894:c246239-245961 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT179777 WP_002892066.1 NZ_CP063943:3529917-3530291 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT179777 NZ_CP084894:c246502-246242 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References