Detailed information of TA system TA179768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1348015..1348657
• Replicon: chromosome
• Accession: NZ_CP063943
• Organism: Klebsiella pneumoniae strain BS329-2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: ISA55_RS06465
• Protein ID: WP_075647414.1
• Coordinates: 1348015..1348188 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ISA55_RS06470
• Protein ID: WP_075647415.1
• Coordinates: 1348247..1348657 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA55_RS06435	1343082..1343996	+	915	WP_040188691.1	conserved phage C-terminal domain-containing protein	-
ISA55_RS06440	1343993..1344802	+	810	WP_075647409.1	Dam family site-specific DNA-(adenine-N6)-methyltransferase	-
ISA55_RS06445	1344827..1345807	+	981	WP_075647410.1	DUF968 domain-containing protein	-
ISA55_RS06450	1345826..1346191	+	366	WP_075647411.1	DUF1133 family protein	-
ISA55_RS06455	1346207..1346623	-	417	WP_130943902.1	hypothetical protein	-
ISA55_RS06460	1346886..1347749	-	864	WP_075647413.1	hypothetical protein	-
ISA55_RS06465	1348015..1348188	+	174	WP_075647414.1	type II toxin-antitoxin system HicA family toxin	Toxin
ISA55_RS06470	1348247..1348657	+	411	WP_075647415.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
ISA55_RS06475	1349503..1349718	+	216	WP_017880269.1	class II holin family protein	-
ISA55_RS06480	1349718..1350215	+	498	WP_075647416.1	lysozyme	-
ISA55_RS06485	1350212..1350562	+	351	WP_016160650.1	hypothetical protein	-
ISA55_RS06490	1351506..1351712	+	207	WP_023159883.1	hypothetical protein	-
ISA55_RS06495	1351696..1352058	+	363	WP_017880264.1	HNH endonuclease	-
ISA55_RS06500	1352010..1352333	+	324	WP_194429326.1	hypothetical protein	-
ISA55_RS06505	1352330..1352761	+	432	WP_081277328.1	hypothetical protein	-
ISA55_RS06510	1353011..1353445	+	435	WP_012542168.1	phage terminase small subunit P27 family	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1338016..1382049	44033

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (86-128)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6603.75 Da
• Isoelectric Point: 11.3260

>T179768 WP_075647414.1 NZ_CP063943:1348015-1348188 [Klebsiella pneumoniae]
VKQREFQRWLESQGVEITDGAKHLKLRLNGKRSVMPRHPGSEIVEQLRKAILKQLGL

Nucleotide

• Length: 174 bp

>T179768 NZ_CP084893:c3046898-3046491 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15269.40 Da
• Isoelectric Point: 4.4249

>AT179768 WP_075647415.1 NZ_CP063943:1348247-1348657 [Klebsiella pneumoniae]
MRYPVNLSKAEEGGFFVEFPDIPEALTQGDTKEEALDNALDALVTAFEFYFEDGDRIPEPGVPTGDYVELPLSIEAKVLM
LNAFIESKLSQTELAAKMNVKKQEVTRLFDLKHSTKIDTIQKAVNSLGHRMELKYA

Nucleotide

• Length: 411 bp

>AT179768 NZ_CP084893:c3047145-3046879 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA