Detailed information of TA system TA179756

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3038769..3040357
• Replicon: chromosome
• Accession: NZ_CP063942
• Organism: Klebsiella quasipneumoniae strain BS418

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: ISA61_RS14360
• Protein ID: WP_130947586.1
• Coordinates: 3039035..3040357 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2A5MFC9
• Locus tag: ISA61_RS14355
• Protein ID: WP_004205921.1
• Coordinates: 3038769..3039035 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA61_RS14330	3033902..3034825	+	924	WP_106939111.1	LysR family hpxDE operon transcriptional regulator HpxR	-
ISA61_RS14335	3034806..3035960	-	1155	WP_004205928.1	FAD-dependent urate hydroxylase HpxO	-
ISA61_RS14340	3036306..3037685	+	1380	WP_017900377.1	purine permease	-
ISA61_RS14345	3037663..3038163	+	501	WP_017900378.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
ISA61_RS14350	3038160..3038486	+	327	WP_069602708.1	hydroxyisourate hydrolase	-
ISA61_RS14355	3038769..3039035	+	267	WP_004205921.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
ISA61_RS14360	3039035..3040357	+	1323	WP_130947586.1	type II toxin-antitoxin system HipA family toxin	Toxin
ISA61_RS14365	3040374..3041666	-	1293	WP_106939108.1	glycoside hydrolase family 10 protein	-
ISA61_RS14370	3041808..3042014	-	207	WP_004205917.1	helix-turn-helix transcriptional regulator	-
ISA61_RS14375	3042017..3042550	-	534	WP_106939107.1	DUF2975 domain-containing protein	-
ISA61_RS14380	3042579..3042923	-	345	WP_004205915.1	hypothetical protein	-
ISA61_RS14385	3042910..3044025	-	1116	WP_130947585.1	serine hydrolase	-
ISA61_RS14390	3044294..3045076	-	783	WP_194418413.1	type I methionyl aminopeptidase	-
ISA61_RS14395	3045057..3045275	-	219	WP_002904808.1	ParD-like family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	blaOKP-B-8	iroE / mgtC	2808843..3209461	400618

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49113.39 Da
• Isoelectric Point: 8.2655

>T179756 WP_130947586.1 NZ_CP063942:3039035-3040357 [Klebsiella quasipneumoniae]
MATLTTWMNNARVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRERIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIINTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILEEFADRVP
QAIESARRTLPSDFSAHVWRAITENMLKLHARLQQGLQAG

Nucleotide

• Length: 1323 bp

>T179756 NZ_CP084893:c1271086-1270979 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9940.38 Da
• Isoelectric Point: 7.1997

>AT179756 WP_004205921.1 NZ_CP063942:3038769-3039035 [Klebsiella quasipneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETAMIP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT179756 NZ_CP084893:1271133-1271199 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Antitoxin

• AlphaFold DB: A0A2A5MFC9