Detailed information of TA system TA179753

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 128757..129493
• Replicon: plasmid pBM343.1
• Accession: NZ_CP063940
• Organism: Klebsiella pneumoniae strain BM343

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: ISA62_RS26740
• Protein ID: WP_016240380.1
• Coordinates: 128757..129239 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: ISA62_RS26745
• Protein ID: WP_003026799.1
• Coordinates: 129227..129493 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA62_RS26725	124818..125813	-	996	WP_049194124.1	IS110 family transposase	-
ISA62_RS26730	126693..127556	+	864	WP_020803592.1	toll/interleukin-1 receptor domain-containing protein	-
ISA62_RS26735	127838..128554	-	717	Protein_144	IS3-like element ISKpn38 family transposase	-
ISA62_RS26740	128757..129239	-	483	WP_016240380.1	GNAT family N-acetyltransferase	Toxin
ISA62_RS26745	129227..129493	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
ISA62_RS26750	129915..131114	-	1200	WP_013609503.1	hypothetical protein	-
ISA62_RS26755	131128..132795	-	1668	WP_115658245.1	hypothetical protein	-
ISA62_RS26760	132818..133327	-	510	WP_013609501.1	SMI1/KNR4 family protein	-
ISA62_RS26765	133540..133776	+	237	Protein_150	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ	1..189015	189015
	-	flank	IS/Tn	-	-	127838..128500	662

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17323.95 Da
• Isoelectric Point: 9.5822

>T179753 WP_016240380.1 NZ_CP063940:c129239-128757 [Klebsiella pneumoniae]
VGRITAPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T179753 NZ_CP084893:c479532-479314 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT179753 WP_003026799.1 NZ_CP063940:c129493-129227 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT179753 NZ_CP084893:c479932-479558 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66