Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3766947..3767572 Replicon chromosome
Accession NZ_CP063939
Organism Klebsiella pneumoniae strain BM343

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag ISA62_RS18140 Protein ID WP_019705794.1
Coordinates 3766947..3767330 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag ISA62_RS18145 Protein ID WP_004150355.1
Coordinates 3767330..3767572 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA62_RS18125 3764313..3765215 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
ISA62_RS18130 3765212..3765847 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
ISA62_RS18135 3765844..3766773 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
ISA62_RS18140 3766947..3767330 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
ISA62_RS18145 3767330..3767572 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
ISA62_RS18150 3767777..3768694 + 918 WP_019705795.1 alpha/beta hydrolase -
ISA62_RS18155 3768708..3769649 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
ISA62_RS18160 3769694..3770131 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
ISA62_RS18165 3770128..3770988 - 861 WP_004146232.1 virulence factor BrkB family protein -
ISA62_RS18170 3770982..3771581 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T179749 WP_019705794.1 NZ_CP063939:c3767330-3766947 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T179749 NZ_CP084892:c2589024-2588758 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTGATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT179749 WP_004150355.1 NZ_CP063939:c3767572-3767330 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT179749 NZ_CP084892:c2589281-2589024 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAGTAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References