Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 3169148..3169958 Replicon chromosome
Accession NZ_CP063939
Organism Klebsiella pneumoniae strain BM343

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag ISA62_RS15390 Protein ID WP_004178461.1
Coordinates 3169148..3169681 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag ISA62_RS15395 Protein ID WP_002887278.1
Coordinates 3169692..3169958 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA62_RS15385 3167979..3169100 + 1122 WP_002887282.1 cupin domain-containing protein -
ISA62_RS15390 3169148..3169681 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
ISA62_RS15395 3169692..3169958 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
ISA62_RS15400 3170061..3171494 - 1434 WP_019705874.1 Cu(+)/Ag(+) sensor histidine kinase -
ISA62_RS15405 3171484..3172167 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
ISA62_RS15410 3172339..3173724 + 1386 WP_032409188.1 efflux transporter outer membrane subunit -
ISA62_RS15415 3173742..3174086 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T179746 WP_004178461.1 NZ_CP063939:c3169681-3169148 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T179746 NZ_CP084892:c2343508-2343404 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCGTGTCTGATTTCGAAATGCTGATGGTTGTATTAACGATTATCAGTTTAGTATTAAT
CAGTAATCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT179746 WP_002887278.1 NZ_CP063939:c3169958-3169692 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT179746 NZ_CP084892:2343282-2343375 [Staphylococcus aureus]
ATAAATAGAAAAAGGGCAACATGCGCTAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCTTTTCATAGAAAAA
TTATAAAAATAACC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References