Detailed information of TA system TA179734

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 138111..138847
• Replicon: plasmid pBS317-1.1
• Accession: NZ_CP063937
• Organism: Klebsiella pneumoniae strain BS317-1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: ISA63_RS25245
• Protein ID: WP_003026803.1
• Coordinates: 138365..138847 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: ISA63_RS25240
• Protein ID: WP_003026799.1
• Coordinates: 138111..138377 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA63_RS25195	133520..133870	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
ISA63_RS25200	133986..134953	+	968	Protein_131	IS5 family transposase	-
ISA63_RS25205	135293..135562	+	270	WP_004152102.1	hypothetical protein	-
ISA63_RS25210	135550..136125	+	576	WP_004152103.1	hypothetical protein	-
ISA63_RS25215	136156..136650	+	495	WP_004152104.1	DNA-binding protein	-
ISA63_RS25220	136694..137062	+	369	WP_004152105.1	hypothetical protein	-
ISA63_RS25225	137096..137299	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
ISA63_RS25230	137348..137605	+	258	WP_004152107.1	hypothetical protein	-
ISA63_RS25235	137681..137935	+	255	WP_004152108.1	hypothetical protein	-
ISA63_RS25240	138111..138377	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
ISA63_RS25245	138365..138847	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
ISA63_RS25250	139008..140262	-	1255	Protein_141	IS3 family transposase	-
ISA63_RS25255	140350..141312	-	963	WP_004152113.1	M48 family metalloprotease	-
ISA63_RS25260	141299..142048	-	750	WP_009483782.1	phosphate-starvation-inducible PsiE family protein	-
ISA63_RS25265	142286..142483	-	198	WP_004152115.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..182144	182144
	-	inside	IScluster/Tn	-	-	133986..140035	6049

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T179734 WP_003026803.1 NZ_CP063937:138365-138847 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T179734 NZ_CP084879:c3628193-3627786 [Shigella flexneri]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGTGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT179734 WP_003026799.1 NZ_CP063937:138111-138377 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT179734 NZ_CP084879:c3628440-3628174 [Shigella flexneri]
ATGGACATTAACAACAAAGCCCGAATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATAATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66