Detailed information of TA system TA179733

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4782447..4784035
• Replicon: chromosome
• Accession: NZ_CP063936
• Organism: Klebsiella pneumoniae strain BS317-1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A486S9S0
• Locus tag: ISA63_RS23175
• Protein ID: WP_023301940.1
• Coordinates: 4782713..4784035 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: ISA63_RS23170
• Protein ID: WP_087787682.1
• Coordinates: 4782447..4782713 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA63_RS23145	4777598..4778521	+	924	WP_080882515.1	LysR family hpxDE operon transcriptional regulator HpxR	-
ISA63_RS23150	4778502..4779656	-	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
ISA63_RS23155	4780002..4781381	+	1380	WP_087787681.1	purine permease	-
ISA63_RS23160	4781359..4781859	+	501	WP_023282573.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
ISA63_RS23165	4781856..4782182	+	327	WP_004215084.1	hydroxyisourate hydrolase	-
ISA63_RS23170	4782447..4782713	+	267	WP_087787682.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
ISA63_RS23175	4782713..4784035	+	1323	WP_023301940.1	type II toxin-antitoxin system HipA family toxin	Toxin
ISA63_RS23180	4784052..4785344	-	1293	WP_087787684.1	glycoside hydrolase family 10 protein	-
ISA63_RS23185	4785486..4786490	-	1005	WP_087787685.1	type 1 fimbrial protein	-
ISA63_RS23190	4786478..4788979	-	2502	WP_087787686.1	fimbrial biogenesis outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49185.49 Da
• Isoelectric Point: 7.5358

>T179733 WP_023301940.1 NZ_CP063936:4782713-4784035 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T179733 NZ_CP084879:c2723018-2722863 [Shigella flexneri]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9950.28 Da
• Isoelectric Point: 7.1999

>AT179733 WP_087787682.1 NZ_CP063936:4782447-4782713 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNDKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT179733 NZ_CP084879:2723030-2723088 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A486S9S0