Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3525949..3526568 Replicon chromosome
Accession NZ_CP063936
Organism Klebsiella pneumoniae strain BS317-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag ISA63_RS17130 Protein ID WP_002892050.1
Coordinates 3525949..3526167 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag ISA63_RS17135 Protein ID WP_002892066.1
Coordinates 3526194..3526568 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA63_RS17095 3521996..3522259 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
ISA63_RS17100 3522259..3522399 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
ISA63_RS17105 3522396..3523094 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
ISA63_RS17110 3523195..3524646 + 1452 WP_087787915.1 PLP-dependent aminotransferase family protein -
ISA63_RS17115 3524621..3525091 - 471 WP_002892026.1 YlaC family protein -
ISA63_RS17120 3525112..3525252 + 141 WP_004147370.1 hypothetical protein -
ISA63_RS17125 3525224..3525790 - 567 WP_002892030.1 maltose O-acetyltransferase -
ISA63_RS17130 3525949..3526167 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
ISA63_RS17135 3526194..3526568 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
ISA63_RS17140 3527054..3530200 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
ISA63_RS17145 3530223..3531416 - 1194 WP_087787916.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T179731 WP_002892050.1 NZ_CP063936:c3526167-3525949 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T179731 NZ_CP084879:2320577-2320864 [Shigella flexneri]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT179731 WP_002892066.1 NZ_CP063936:c3526568-3526194 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT179731 NZ_CP084879:2320338-2320577 [Shigella flexneri]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References