Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 2932694..2933504 Replicon chromosome
Accession NZ_CP063936
Organism Klebsiella pneumoniae strain BS317-1

Toxin (Protein)


Gene name KacT Uniprot ID -
Locus tag ISA63_RS14275 Protein ID WP_087788033.1
Coordinates 2932971..2933504 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag ISA63_RS14270 Protein ID WP_002887278.1
Coordinates 2932694..2932960 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA63_RS14250 2928566..2928910 - 345 WP_087788039.1 cation efflux system protein CusF -
ISA63_RS14255 2928928..2930313 - 1386 WP_087788035.1 efflux transporter outer membrane subunit -
ISA63_RS14260 2930485..2931168 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
ISA63_RS14265 2931158..2932591 + 1434 WP_087788034.1 Cu(+)/Ag(+) sensor histidine kinase -
ISA63_RS14270 2932694..2932960 + 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
ISA63_RS14275 2932971..2933504 + 534 WP_087788033.1 GNAT family N-acetyltransferase Toxin
ISA63_RS14280 2933552..2934673 - 1122 WP_004214138.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19844.67 Da        Isoelectric Point: 5.2614

>T179730 WP_087788033.1 NZ_CP063936:2932971-2933504 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRFLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T179730 NZ_CP084879:2137503-2137658 [Shigella flexneri]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT179730 WP_002887278.1 NZ_CP063936:2932694-2932960 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT179730 NZ_CP084879:c2137491-2137433 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References