Detailed information of TA system TA179728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 2657339..2659020
• Replicon: chromosome
• Accession: NZ_CP063936
• Organism: Klebsiella pneumoniae strain BS317-1

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: ISA63_RS12965
• Protein ID: WP_087787586.1
• Coordinates: 2658103..2659020 (+)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: ISA63_RS12960
• Protein ID: WP_087787585.1
• Coordinates: 2657339..2658097 (+)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA63_RS12945	2653189..2653875	-	687	WP_004151733.1	sel1 repeat family protein	-
ISA63_RS12950	2654105..2656252	-	2148	WP_087787584.1	formate dehydrogenase subunit alpha	-
ISA63_RS12955	2656525..2657253	+	729	WP_002885152.1	response regulator transcription factor	-
ISA63_RS12960	2657339..2658097	+	759	WP_087787585.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
ISA63_RS12965	2658103..2659020	+	918	WP_087787586.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
ISA63_RS12970	2659145..2660152	-	1008	WP_004178300.1	carbohydrate kinase family protein	-
ISA63_RS12975	2660227..2661168	-	942	WP_087787587.1	ABC transporter substrate-binding protein	-
ISA63_RS12980	2661199..2662197	-	999	WP_002885172.1	ribose ABC transporter permease	-
ISA63_RS12985	2662194..2663714	-	1521	WP_004210021.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-246)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34697.17 Da
• Isoelectric Point: 5.4707

>T179728 WP_087787586.1 NZ_CP063936:2658103-2659020 [Klebsiella pneumoniae]
IDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIVYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIVALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T179728 NZ_CP084879:1485314-1485544 [Shigella flexneri]
ATGAAGGTATACGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGGCTTTTCGAACTCAGAATTAAAGG
CAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTGCATAGTTTTGTAAAGAAAG
CGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCATGACTGGTAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28576.98 Da
• Isoelectric Point: 10.0870

>AT179728 WP_087787585.1 NZ_CP063936:2657339-2658097 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYVRAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT179728 NZ_CP084879:1485534-1485842 [Shigella flexneri]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACACTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA