Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 58004..58529 Replicon plasmid pBS326-3
Accession NZ_CP063935
Organism Klebsiella pneumoniae strain BS326-3

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag ISA64_RS25760 Protein ID WP_004197633.1
Coordinates 58224..58529 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag ISA64_RS25755 Protein ID WP_006788213.1
Coordinates 58004..58222 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA64_RS25735 53907..55184 - 1278 Protein_59 HlyD family secretion protein -
ISA64_RS25740 55845..56723 + 879 WP_064182004.1 restriction endonuclease -
ISA64_RS25745 56764..57180 - 417 WP_186474563.1 type II toxin-antitoxin system VapC family toxin -
ISA64_RS25750 57177..57407 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
ISA64_RS25755 58004..58222 + 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ISA64_RS25760 58224..58529 + 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
ISA64_RS25765 58697..59098 + 402 WP_072199347.1 hypothetical protein -
ISA64_RS25770 59125..59448 + 324 WP_004197641.1 hypothetical protein -
ISA64_RS25775 59445..60461 + 1017 WP_194429503.1 hypothetical protein -
ISA64_RS25780 60659..61438 + 780 WP_194429504.1 site-specific integrase -
ISA64_RS25785 61496..61753 - 258 WP_194429505.1 hypothetical protein -
ISA64_RS25790 61882..61995 - 114 WP_014343462.1 hypothetical protein -
ISA64_RS25795 62570..63397 + 828 WP_194429506.1 replication initiation protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) - 1..104836 104836


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T179716 WP_004197633.1 NZ_CP063935:58224-58529 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T179716 NZ_CP084876:c4789985-4789701 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGACCTATGAACTGGCGTTTGATCCGCGTGCCTGGCGCGAATGGCATAAGCTTGGCGAGACGATCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGGTTGTGCAGAATCCGCGAATTGCATCGGCCAGTCTGAGTGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGGGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT179716 WP_006788213.1 NZ_CP063935:58004-58222 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT179716 NZ_CP084876:c4790217-4789975 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGGCCACGCTCAACGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTGCGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCAGTGAAAAGTATGACTATCAGCGATA
GCGAAGCGGCATTATTACGCACCGTCCGCGAGCGGCTGGAAAACCCGCAGCCGGAGGTGAAGGTAAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References