Detailed information of TA system TA179715

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 56764..57407
• Replicon: plasmid pBS326-3
• Accession: NZ_CP063935
• Organism: Klebsiella pneumoniae strain BS326-3

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: ISA64_RS25745
• Protein ID: WP_186474563.1
• Coordinates: 56764..57180 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: ISA64_RS25750
• Protein ID: WP_001261276.1
• Coordinates: 57177..57407 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA64_RS25730	51809..53917	-	2109	WP_114473038.1	peptidase domain-containing ABC transporter	-
ISA64_RS25735	53907..55184	-	1278	Protein_59	HlyD family secretion protein	-
ISA64_RS25740	55845..56723	+	879	WP_064182004.1	restriction endonuclease	-
ISA64_RS25745	56764..57180	-	417	WP_186474563.1	type II toxin-antitoxin system VapC family toxin	Toxin
ISA64_RS25750	57177..57407	-	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ISA64_RS25755	58004..58222	+	219	WP_006788213.1	type II toxin-antitoxin system antitoxin CcdA	-
ISA64_RS25760	58224..58529	+	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	-
ISA64_RS25765	58697..59098	+	402	WP_072199347.1	hypothetical protein	-
ISA64_RS25770	59125..59448	+	324	WP_004197641.1	hypothetical protein	-
ISA64_RS25775	59445..60461	+	1017	WP_194429503.1	hypothetical protein	-
ISA64_RS25780	60659..61438	+	780	WP_194429504.1	site-specific integrase	-
ISA64_RS25785	61496..61753	-	258	WP_194429505.1	hypothetical protein	-
ISA64_RS25790	61882..61995	-	114	WP_014343462.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A)	-	1..104836	104836

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15078.58 Da
• Isoelectric Point: 8.5403

>T179715 WP_186474563.1 NZ_CP063935:c57180-56764 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T179715 NZ_CP084876:4064392-4064610 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGCCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT179715 WP_001261276.1 NZ_CP063935:c57407-57177 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT179715 NZ_CP084876:4063991-4064365 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGATACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A387K023