Detailed information of TA system TA179704

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2704232..2704874
• Replicon: chromosome
• Accession: NZ_CP063934
• Organism: Klebsiella pneumoniae strain BS326-3

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: ISA64_RS12940
• Protein ID: WP_075647414.1
• Coordinates: 2704232..2704405 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ISA64_RS12945
• Protein ID: WP_075647415.1
• Coordinates: 2704464..2704874 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA64_RS12910	2699299..2700213	+	915	WP_040188691.1	conserved phage C-terminal domain-containing protein	-
ISA64_RS12915	2700210..2701019	+	810	WP_075647409.1	Dam family site-specific DNA-(adenine-N6)-methyltransferase	-
ISA64_RS12920	2701044..2702024	+	981	WP_075647410.1	DUF968 domain-containing protein	-
ISA64_RS12925	2702043..2702408	+	366	WP_075647411.1	DUF1133 family protein	-
ISA64_RS12930	2702424..2702840	-	417	WP_130943902.1	hypothetical protein	-
ISA64_RS12935	2703103..2703966	-	864	WP_075647413.1	hypothetical protein	-
ISA64_RS12940	2704232..2704405	+	174	WP_075647414.1	type II toxin-antitoxin system HicA family toxin	Toxin
ISA64_RS12945	2704464..2704874	+	411	WP_075647415.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
ISA64_RS12950	2705720..2705935	+	216	WP_017880269.1	class II holin family protein	-
ISA64_RS12955	2705935..2706432	+	498	WP_075647416.1	lysozyme	-
ISA64_RS12960	2706429..2706779	+	351	WP_016160650.1	hypothetical protein	-
ISA64_RS12965	2707723..2707929	+	207	WP_023159883.1	hypothetical protein	-
ISA64_RS12970	2707913..2708275	+	363	WP_017880264.1	HNH endonuclease	-
ISA64_RS12975	2708227..2708550	+	324	WP_194429326.1	hypothetical protein	-
ISA64_RS12980	2708547..2708978	+	432	WP_081277328.1	hypothetical protein	-
ISA64_RS12985	2709228..2709662	+	435	WP_012542168.1	phage terminase small subunit P27 family	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2694233..2738266	44033

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (86-128)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6603.75 Da
• Isoelectric Point: 11.3260

>T179704 WP_075647414.1 NZ_CP063934:2704232-2704405 [Klebsiella pneumoniae]
VKQREFQRWLESQGVEITDGAKHLKLRLNGKRSVMPRHPGSEIVEQLRKAILKQLGL

Nucleotide

• Length: 174 bp

>T179704 NZ_CP084874:1885092-1885194 [Klebsiella africana]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15269.40 Da
• Isoelectric Point: 4.4249

>AT179704 WP_075647415.1 NZ_CP063934:2704464-2704874 [Klebsiella pneumoniae]
MRYPVNLSKAEEGGFFVEFPDIPEALTQGDTKEEALDNALDALVTAFEFYFEDGDRIPEPGVPTGDYVELPLSIEAKVLM
LNAFIESKLSQTELAAKMNVKKQEVTRLFDLKHSTKIDTIQKAVNSLGHRMELKYA

Nucleotide

• Length: 411 bp

>AT179704 NZ_CP084874:c1885196-1885056 [Klebsiella africana]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT