Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2654940..2655530 Replicon chromosome
Accession NZ_CP063934
Organism Klebsiella pneumoniae strain BS326-3

Toxin (Protein)


Gene name pemK Uniprot ID A0A2L1BXX2
Locus tag ISA64_RS12640 Protein ID WP_022615588.1
Coordinates 2655198..2655530 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag ISA64_RS12635 Protein ID WP_000288812.1
Coordinates 2654940..2655197 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA64_RS12615 2651124..2652359 - 1236 WP_021466313.1 DNA (cytosine-5-)-methyltransferase -
ISA64_RS12620 2652631..2653632 + 1002 WP_032412597.1 DUF4917 family protein -
ISA64_RS12625 2653941..2654402 + 462 WP_032412594.1 hypothetical protein -
ISA64_RS12630 2654399..2654605 + 207 WP_032412591.1 helix-turn-helix domain-containing protein -
ISA64_RS12635 2654940..2655197 + 258 WP_000288812.1 antitoxin Antitoxin
ISA64_RS12640 2655198..2655530 + 333 WP_022615588.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ISA64_RS12650 2655852..2657288 + 1437 WP_004151469.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
ISA64_RS12660 2657654..2659108 - 1455 WP_023301083.1 AMP nucleosidase -
ISA64_RS12665 2659238..2659483 - 246 WP_004141189.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2646461..2655530 9069


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11913.80 Da        Isoelectric Point: 10.1839

>T179703 WP_022615588.1 NZ_CP063934:2655198-2655530 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDVVVNEVLARLDAMLS

Download         Length: 333 bp

>T179703 NZ_CP084874:c1783797-1783444 [Klebsiella africana]
GTGTGGACGATTAAAACAACGGATATGTTTGATCGCTGGTTTACCTCGCTCAATGATATCGATCGGGCCAGCGTGCTGGC
AGCGCTGTTGGTCTTGCGGGAAAAGGGGCCGGGTTTGTCCAGACCTTATGCCGATACGATCAGAGGTTCCCGCTACAGCA
ACATGAAAGAGTTGCGTGTTCAGAGCCGGGGCGACCCAATACGGGCGTTTTTTGCTTTTGACCCGGCCCGTACCGGCATT
GTGCTTTGTGCCGGAAATAAGGTCGGCAATGAAAAACGGTTTTACCATGAGATGCTCGCGGTCGCAGATCGGGAATTCAC
GAACTGGCTTAACAGATTGAAAGAGAAGGAGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT179703 WP_000288812.1 NZ_CP063934:2654940-2655197 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT179703 NZ_CP084874:c1783439-1783137 [Klebsiella africana]
ATGGGCAGAACGCTGGAACAGCTTCTTATAGATGAAAAACCGGATGTCGTTGCCGAAGCTCAGGCTATTGCTAAGGATAT
CCTGCTTAATATTCATCTTGCCGAACTACGTGAGAAGGTGCAGAAGACCCAGGTAGAAATGGCTCAGGATTTAGGCATAA
AACAGCCTACCGTTGCAGGAATGGAAAAACCGGGCCGCGACCTGAAGCTCTCTACCCTTAAGCGTTATGTTGAAGCGGCG
GGTGGTAAGCTGCGGCTTGATGTTGAGCTCCCGGACGGGTCGCACTATGAGTTTGTTTTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1BXX2

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References