Detailed information of TA system TA179692

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3654609..3656290
• Replicon: chromosome
• Accession: NZ_CP063933
• Organism: Klebsiella variicola strain BS369-2

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A1F2M1V2
• Locus tag: ISA65_RS17740
• Protein ID: WP_032734038.1
• Coordinates: 3655373..3656290 (+)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: ISA65_RS17735
• Protein ID: WP_032734037.1
• Coordinates: 3654609..3655367 (+)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA65_RS17720	3650336..3651022	-	687	WP_016160120.1	sel1 repeat family protein	-
ISA65_RS17725	3651376..3653523	-	2148	WP_194387313.1	formate dehydrogenase subunit alpha	-
ISA65_RS17730	3653796..3654524	+	729	WP_194387312.1	response regulator transcription factor	-
ISA65_RS17735	3654609..3655367	+	759	WP_032734037.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
ISA65_RS17740	3655373..3656290	+	918	WP_032734038.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
ISA65_RS17745	3656415..3657422	-	1008	WP_022065799.1	carbohydrate kinase family protein	-
ISA65_RS17750	3657497..3658438	-	942	WP_012543155.1	ABC transporter substrate-binding protein	-
ISA65_RS17755	3658469..3659467	-	999	WP_004206363.1	ribose ABC transporter permease	-
ISA65_RS17760	3659464..3660984	-	1521	WP_064324014.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-246)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34733.10 Da
• Isoelectric Point: 5.4707

>T179692 WP_032734038.1 NZ_CP063933:3655373-3656290 [Klebsiella variicola]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRTIALPHIRDALARIAANLQQQ
AGINAVLQANRSDEMRVVVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELNRPIFNGFIAYLLSHNRPLAEVLSPRWKDIAEPFYREFSGMTFETIALEELTAVPYRMIDALKS
CFTQQDIDFLLSFKRGEPDWRLAPETRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T179692 NZ_CP084873:1856874-1856976 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28693.08 Da
• Isoelectric Point: 10.2152

>AT179692 WP_032734037.1 NZ_CP063933:3654609-3655367 [Klebsiella variicola]
MPSKLNWLLQNSAPGDVILQSWLSRHAISPSLAFKYTQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSMENKALLPKWFREFPGVEWIVISGQKLPVLDDRYRVLLDVKGKRLTGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQTKRLYLFFASFYEHGWLRRIDSQKIDLGAGKRQIVVNGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT179692 NZ_CP084873:c1856983-1856838 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Toxin

• AlphaFold DB: A0A1F2M1V2