Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3330807..3331432 Replicon chromosome
Accession NZ_CP063933
Organism Klebsiella variicola strain BS369-2

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag ISA65_RS16240 Protein ID WP_008807903.1
Coordinates 3331049..3331432 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag ISA65_RS16235 Protein ID WP_004150355.1
Coordinates 3330807..3331049 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA65_RS16210 3326797..3327396 + 600 WP_008807908.1 glucose-1-phosphatase -
ISA65_RS16215 3327390..3328250 + 861 WP_008807907.1 virulence factor BrkB family protein -
ISA65_RS16220 3328247..3328684 + 438 WP_043875980.1 D-tyrosyl-tRNA(Tyr) deacylase -
ISA65_RS16225 3328729..3329670 + 942 WP_022064599.1 fatty acid biosynthesis protein FabY -
ISA65_RS16230 3329685..3330602 - 918 WP_043875979.1 alpha/beta hydrolase -
ISA65_RS16235 3330807..3331049 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
ISA65_RS16240 3331049..3331432 + 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
ISA65_RS16245 3331606..3332535 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
ISA65_RS16250 3332532..3333167 - 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
ISA65_RS16255 3333164..3334066 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T179691 WP_008807903.1 NZ_CP063933:3331049-3331432 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T179691 NZ_CP084873:801317-801727 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT179691 WP_004150355.1 NZ_CP063933:3330807-3331049 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT179691 NZ_CP084873:801070-801336 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References