Detailed information of TA system TA179690

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 2936423..2937009
• Replicon: chromosome
• Accession: NZ_CP063933
• Organism: Klebsiella variicola strain BS369-2

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: ISA65_RS14435
• Protein ID: WP_002920800.1
• Coordinates: 2936423..2936791 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A1F2M0C2
• Locus tag: ISA65_RS14440
• Protein ID: WP_032731361.1
• Coordinates: 2936788..2937009 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA65_RS14415	2931907..2932977	-	1071	WP_023323353.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-
ISA65_RS14420	2932979..2933824	-	846	WP_032740290.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
ISA65_RS14425	2933821..2934708	-	888	WP_012967120.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
ISA65_RS14430	2934815..2936131	-	1317	WP_194386930.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
ISA65_RS14435	2936423..2936791	-	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
ISA65_RS14440	2936788..2937009	-	222	WP_032731361.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ISA65_RS14445	2937173..2937886	-	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
ISA65_RS14450	2937888..2938655	-	768	WP_008806972.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
ISA65_RS14455	2938652..2939929	-	1278	WP_008806971.1	branched chain amino acid ABC transporter permease LivM	-
ISA65_RS14460	2939926..2940852	-	927	WP_012540389.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2931170..2951903	20733

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T179690 WP_002920800.1 NZ_CP063933:c2936791-2936423 [Klebsiella variicola]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T179690 NZ_CP084873:359865-360212 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8165.18 Da
• Isoelectric Point: 4.7769

>AT179690 WP_032731361.1 NZ_CP063933:c2937009-2936788 [Klebsiella variicola]
MRTVNYSEARQNLADVLESAVTGIPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT179690 NZ_CP084873:360212-360511 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A1F2M0C2