Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2433852..2434509 Replicon chromosome
Accession NZ_CP063933
Organism Klebsiella variicola strain BS369-2

Toxin (Protein)


Gene name cptA Uniprot ID W8UCT0
Locus tag ISA65_RS11815 Protein ID WP_002916310.1
Coordinates 2433852..2434262 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag ISA65_RS11820 Protein ID WP_002916312.1
Coordinates 2434243..2434509 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA65_RS11795 2429852..2431585 - 1734 WP_008806424.1 single-stranded-DNA-specific exonuclease RecJ -
ISA65_RS11800 2431591..2432304 - 714 WP_194386987.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ISA65_RS11805 2432327..2433223 - 897 WP_194386986.1 site-specific tyrosine recombinase XerD -
ISA65_RS11810 2433324..2433845 + 522 WP_008806427.1 flavodoxin FldB -
ISA65_RS11815 2433852..2434262 - 411 WP_002916310.1 protein YgfX Toxin
ISA65_RS11820 2434243..2434509 - 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
ISA65_RS11825 2434755..2435738 + 984 WP_043876073.1 tRNA-modifying protein YgfZ -
ISA65_RS11830 2435785..2436123 - 339 WP_194386985.1 hypothetical protein -
ISA65_RS11835 2436258..2436917 - 660 WP_043876072.1 hemolysin III family protein -
ISA65_RS11840 2437081..2437392 - 312 WP_012540591.1 N(4)-acetylcytidine aminohydrolase -
ISA65_RS11845 2437443..2438171 + 729 WP_012967245.1 MurR/RpiR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16049.85 Da        Isoelectric Point: 11.4778

>T179688 WP_002916310.1 NZ_CP063933:c2434262-2433852 [Klebsiella variicola]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 411 bp

>T179688 NZ_CP084873:202692-203177 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAACCAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTACTTCGTTGCTATCGGGTTGCCGAGAATATAGGTGTACGTGCAATCATGGTTCATGCGCTCACAG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCCGCGGACACTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT179688 WP_002916312.1 NZ_CP063933:c2434509-2434243 [Klebsiella variicola]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT179688 NZ_CP084873:202438-202704 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAGCTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GSW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References