Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5491674..5492299 Replicon chromosome
Accession NZ_CP063932
Organism Klebsiella variicola strain BS375-3

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag ISA66_RS26470 Protein ID WP_008807903.1
Coordinates 5491916..5492299 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag ISA66_RS26465 Protein ID WP_004150355.1
Coordinates 5491674..5491916 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA66_RS26440 5487664..5488263 + 600 WP_008807908.1 glucose-1-phosphatase -
ISA66_RS26445 5488257..5489117 + 861 WP_008807907.1 virulence factor BrkB family protein -
ISA66_RS26450 5489114..5489551 + 438 WP_043875980.1 D-tyrosyl-tRNA(Tyr) deacylase -
ISA66_RS26455 5489596..5490537 + 942 WP_022064599.1 fatty acid biosynthesis protein FabY -
ISA66_RS26460 5490552..5491469 - 918 WP_043875979.1 alpha/beta hydrolase -
ISA66_RS26465 5491674..5491916 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
ISA66_RS26470 5491916..5492299 + 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
ISA66_RS26475 5492473..5493402 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
ISA66_RS26480 5493399..5494034 - 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
ISA66_RS26485 5494031..5494933 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T179677 WP_008807903.1 NZ_CP063932:5491916-5492299 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T179677 NZ_CP084870:c5002978-5002493 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGATAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT179677 WP_004150355.1 NZ_CP063932:5491674-5491916 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT179677 NZ_CP084870:c5003268-5002975 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References