Detailed information of TA system TA179676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 5097293..5097879
• Replicon: chromosome
• Accession: NZ_CP063932
• Organism: Klebsiella variicola strain BS375-3

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: ISA66_RS24665
• Protein ID: WP_002920800.1
• Coordinates: 5097293..5097661 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A1F2M0C2
• Locus tag: ISA66_RS24670
• Protein ID: WP_032731361.1
• Coordinates: 5097658..5097879 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA66_RS24645	5092777..5093847	-	1071	WP_023323353.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-
ISA66_RS24650	5093849..5094694	-	846	WP_032740290.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
ISA66_RS24655	5094691..5095578	-	888	WP_012967120.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
ISA66_RS24660	5095685..5097001	-	1317	WP_194386930.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
ISA66_RS24665	5097293..5097661	-	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
ISA66_RS24670	5097658..5097879	-	222	WP_032731361.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ISA66_RS24675	5098043..5098756	-	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
ISA66_RS24680	5098758..5099525	-	768	WP_008806972.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
ISA66_RS24685	5099522..5100799	-	1278	WP_008806971.1	branched chain amino acid ABC transporter permease LivM	-
ISA66_RS24690	5100796..5101722	-	927	WP_012540389.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5092040..5112773	20733

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T179676 WP_002920800.1 NZ_CP063932:c5097661-5097293 [Klebsiella variicola]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T179676 NZ_CP084870:c4921195-4920785 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATTCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8165.18 Da
• Isoelectric Point: 4.7769

>AT179676 WP_032731361.1 NZ_CP063932:c5097879-5097658 [Klebsiella variicola]
MRTVNYSEARQNLADVLESAVTGIPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT179676 NZ_CP084870:c4921442-4921176 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A1F2M0C2