Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3585974..3586564 Replicon chromosome
Accession NZ_CP063932
Organism Klebsiella variicola strain BS375-3

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag ISA66_RS17305 Protein ID WP_008804165.1
Coordinates 3585974..3586306 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag ISA66_RS17310 Protein ID WP_012541132.1
Coordinates 3586307..3586564 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA66_RS17280 3582012..3582257 + 246 WP_008804171.1 signal transduction protein PmrD -
ISA66_RS17285 3582388..3583842 + 1455 WP_008804170.1 AMP nucleosidase -
ISA66_RS17295 3584215..3585651 - 1437 WP_016161429.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
ISA66_RS17305 3585974..3586306 - 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ISA66_RS17310 3586307..3586564 - 258 WP_012541132.1 antitoxin Antitoxin
ISA66_RS17315 3587093..3588193 + 1101 WP_110202033.1 phosphotransferase -
ISA66_RS17320 3588190..3589161 - 972 WP_012541130.1 sensor domain-containing diguanylate cyclase -
ISA66_RS17325 3589368..3590204 - 837 WP_008804155.1 alpha/beta hydrolase -
ISA66_RS17330 3590381..3590956 - 576 WP_043875014.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T179673 WP_008804165.1 NZ_CP063932:c3586306-3585974 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T179673 NZ_CP084870:1858050-1858367 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAAGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT179673 WP_012541132.1 NZ_CP063932:c3586564-3586307 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT179673 NZ_CP084870:1858360-1858647 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References