Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1173648..1174267 Replicon chromosome
Accession NZ_CP063932
Organism Klebsiella variicola strain BS375-3

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag ISA66_RS05495 Protein ID WP_002892050.1
Coordinates 1173648..1173866 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag ISA66_RS05500 Protein ID WP_008805436.1
Coordinates 1173893..1174267 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA66_RS05460 1169691..1169954 + 264 WP_008805431.1 type B 50S ribosomal protein L31 -
ISA66_RS05465 1169954..1170094 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
ISA66_RS05470 1170091..1170789 - 699 WP_016160391.1 GNAT family N-acetyltransferase -
ISA66_RS05475 1170890..1172347 + 1458 WP_194387274.1 PLP-dependent aminotransferase family protein -
ISA66_RS05480 1172316..1172786 - 471 WP_008805434.1 YlaC family protein -
ISA66_RS05485 1172823..1172951 + 129 Protein_1051 hypothetical protein -
ISA66_RS05490 1172923..1173489 - 567 WP_008805435.1 maltose O-acetyltransferase -
ISA66_RS05495 1173648..1173866 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
ISA66_RS05500 1173893..1174267 - 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
ISA66_RS05505 1174750..1177896 - 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit -
ISA66_RS05510 1177919..1179112 - 1194 WP_008805438.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T179662 WP_002892050.1 NZ_CP063932:c1173866-1173648 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T179662 NZ_CP084868:c4822830-4822420 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT179662 WP_008805436.1 NZ_CP063932:c1174267-1173893 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT179662 NZ_CP084868:c4823077-4822811 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References