Detailed information of TA system TA179661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 298218..299899
• Replicon: chromosome
• Accession: NZ_CP063932
• Organism: Klebsiella variicola strain BS375-3

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A1F2M1V2
• Locus tag: ISA66_RS01375
• Protein ID: WP_032734038.1
• Coordinates: 298982..299899 (+)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: ISA66_RS01370
• Protein ID: WP_032734037.1
• Coordinates: 298218..298976 (+)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA66_RS01355	293945..294631	-	687	WP_016160120.1	sel1 repeat family protein	-
ISA66_RS01360	294985..297132	-	2148	WP_194387313.1	formate dehydrogenase subunit alpha	-
ISA66_RS01365	297405..298133	+	729	WP_194387312.1	response regulator transcription factor	-
ISA66_RS01370	298218..298976	+	759	WP_032734037.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
ISA66_RS01375	298982..299899	+	918	WP_032734038.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
ISA66_RS01380	300024..301031	-	1008	WP_022065799.1	carbohydrate kinase family protein	-
ISA66_RS01385	301106..302047	-	942	WP_012543155.1	ABC transporter substrate-binding protein	-
ISA66_RS01390	302078..303076	-	999	WP_004206363.1	ribose ABC transporter permease	-
ISA66_RS01395	303073..304593	-	1521	WP_064324014.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-246)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34733.10 Da
• Isoelectric Point: 5.4707

>T179661 WP_032734038.1 NZ_CP063932:298982-299899 [Klebsiella variicola]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRTIALPHIRDALARIAANLQQQ
AGINAVLQANRSDEMRVVVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELNRPIFNGFIAYLLSHNRPLAEVLSPRWKDIAEPFYREFSGMTFETIALEELTAVPYRMIDALKS
CFTQQDIDFLLSFKRGEPDWRLAPETRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T179661 NZ_CP084868:c3791645-3791313 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28693.08 Da
• Isoelectric Point: 10.2152

>AT179661 WP_032734037.1 NZ_CP063932:298218-298976 [Klebsiella variicola]
MPSKLNWLLQNSAPGDVILQSWLSRHAISPSLAFKYTQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSMENKALLPKWFREFPGVEWIVISGQKLPVLDDRYRVLLDVKGKRLTGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQTKRLYLFFASFYEHGWLRRIDSQKIDLGAGKRQIVVNGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT179661 NZ_CP084868:c3791903-3791646 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTAGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Toxin

• AlphaFold DB: A0A1F2M1V2