Detailed information of TA system TA179658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 152345..153084
• Replicon: plasmid pM186-2
• Accession: NZ_CP063931
• Organism: Klebsiella pneumoniae strain M186-2

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: ISA67_RS25980
• Protein ID: WP_021312536.1
• Coordinates: 152599..153084 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: ISA67_RS25975
• Protein ID: WP_003026799.1
• Coordinates: 152345..152611 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA67_RS25940	147452..147922	+	471	WP_004144067.1	RES family NAD+ phosphorylase	-
ISA67_RS25945	148033..148293	-	261	WP_048291871.1	hypothetical protein	-
ISA67_RS25950	148989..149498	+	510	WP_004901294.1	IS3 family transposase	-
ISA67_RS25955	149453..150268	+	816	WP_077268817.1	IS3 family transposase	-
ISA67_RS25965	151437..151583	+	147	WP_159193595.1	IS3 family transposase	-
ISA67_RS25970	151784..152212	+	429	WP_004901287.1	GFA family protein	-
ISA67_RS25975	152345..152611	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
ISA67_RS25980	152599..153084	+	486	WP_021312536.1	GNAT family N-acetyltransferase	Toxin
ISA67_RS25985	153427..153588	+	162	WP_004118124.1	type I toxin-antitoxin system Hok family toxin	-
ISA67_RS25990	154193..155167	+	975	WP_048291873.1	hypothetical protein	-
ISA67_RS25995	155364..155741	-	378	WP_032719541.1	hypothetical protein	-
ISA67_RS26000	155975..156271	+	297	WP_048291874.1	hypothetical protein	-
ISA67_RS26005	156340..157407	+	1068	WP_048291875.1	hypothetical protein	-
ISA67_RS26010	157503..157940	+	438	WP_126034224.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fyuA / ybtE / ybtT / ybtU / irp1 / irp2 / irp2 / ybtA / ybtP / ybtQ / ybtX / ybtS / iroB / iroC / iroD / iroN / rmpA	1..191041	191041

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17608.39 Da
• Isoelectric Point: 10.3370

>T179658 WP_021312536.1 NZ_CP063931:152599-153084 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T179658 NZ_CP084868:c1575026-1574808 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT179658 WP_003026799.1 NZ_CP063931:152345-152611 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT179658 NZ_CP084868:c1575427-1575053 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66