Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 147012..147922 Replicon plasmid pM186-2
Accession NZ_CP063931
Organism Klebsiella pneumoniae strain M186-2

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag ISA67_RS25940 Protein ID WP_004144067.1
Coordinates 147452..147922 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag ISA67_RS25935 Protein ID WP_004026357.1
Coordinates 147012..147455 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA67_RS25895 142251..142479 - 229 Protein_126 type II toxin-antitoxin system Phd/YefM family antitoxin -
ISA67_RS25900 142558..142782 - 225 WP_127807699.1 DUF4113 domain-containing protein -
ISA67_RS25905 142712..143086 - 375 WP_173675457.1 hypothetical protein -
ISA67_RS25910 143141..144186 - 1046 Protein_129 IS3 family transposase -
ISA67_RS25915 144581..144922 - 342 WP_048291869.1 hypothetical protein -
ISA67_RS25920 144922..145659 - 738 Protein_131 DUF1311 domain-containing protein -
ISA67_RS25925 146083..146304 + 222 WP_072060588.1 DUF2188 domain-containing protein -
ISA67_RS25930 146405..146911 + 507 Protein_133 DUF4113 domain-containing protein -
ISA67_RS25935 147012..147455 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
ISA67_RS25940 147452..147922 + 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
ISA67_RS25945 148033..148293 - 261 WP_048291871.1 hypothetical protein -
ISA67_RS25950 148989..149498 + 510 WP_004901294.1 IS3 family transposase -
ISA67_RS25955 149453..150268 + 816 WP_077268817.1 IS3 family transposase -
ISA67_RS25965 151437..151583 + 147 WP_159193595.1 IS3 family transposase -
ISA67_RS25970 151784..152212 + 429 WP_004901287.1 GFA family protein -
ISA67_RS25975 152345..152611 + 267 WP_003026799.1 DUF1778 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - fyuA / ybtE / ybtT / ybtU / irp1 / irp2 / irp2 / ybtA / ybtP / ybtQ / ybtX / ybtS / iroB / iroC / iroD / iroN / rmpA 1..191041 191041


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T179657 WP_004144067.1 NZ_CP063931:147452-147922 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T179657 NZ_CP084868:967876-968409 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT179657 WP_004026357.1 NZ_CP063931:147012-147455 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT179657 NZ_CP084868:967599-967865 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References