Detailed information of TA system TA179656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 116947..117698
• Replicon: plasmid pM186-2
• Accession: NZ_CP063931
• Organism: Klebsiella pneumoniae strain M186-2

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: ISA67_RS25750
• Protein ID: WP_048333033.1
• Coordinates: 116947..117429 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: ISA67_RS25755
• Protein ID: WP_004902250.1
• Coordinates: 117420..117698 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA67_RS25730	113346..113993	-	648	WP_014386537.1	EcsC family protein	-
ISA67_RS25735	114020..114775	-	756	WP_004902235.1	DUF2971 domain-containing protein	-
ISA67_RS25740	114876..115268	-	393	WP_032442757.1	hypothetical protein	-
ISA67_RS25745	115373..115912	-	540	WP_004902239.1	hypothetical protein	-
ISA67_RS25750	116947..117429	-	483	WP_048333033.1	GNAT family N-acetyltransferase	Toxin
ISA67_RS25755	117420..117698	-	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
ISA67_RS25760	117820..118032	-	213	WP_194444813.1	hypothetical protein	-
ISA67_RS25765	118140..118481	-	342	WP_032437747.1	hypothetical protein	-
ISA67_RS25770	119387..119701	+	315	WP_040210113.1	hypothetical protein	-
ISA67_RS25775	119944..120402	-	459	WP_173675456.1	hypothetical protein	-
ISA67_RS25780	121654..122538	+	885	WP_004186900.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fyuA / ybtE / ybtT / ybtU / irp1 / irp2 / irp2 / ybtA / ybtP / ybtQ / ybtX / ybtS / iroB / iroC / iroD / iroN / rmpA	1..191041	191041

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17708.53 Da
• Isoelectric Point: 8.9130

>T179656 WP_048333033.1 NZ_CP063931:c117429-116947 [Klebsiella pneumoniae]
MGMRAPESLMSEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T179656 NZ_CP084868:c913905-912970 [Klebsiella pneumoniae]
CTGCTTTTTTCTGATGTAACAGATGCTCTTGGTATTGAGTCCGGCGCTATTGTAGAAAAAGATCACTATATCGTTGAGCT
TTTACATTTACTGAAACCGCTAACATTTGACACTCATCAATTGCTGTTTGCCGGAGGAACTGCACTGGCAAAATCGGGCA
TTGTGCTTAACCGAATGTCGGAAGATGTAGATATAAAACTGGTACCCACAGATACTTTCTTGTCTCTTTCGCGCACCCAG
CGCAAAGGTATTCGTAAAGAGATCGTCCAGACAATTTCTCATGCAATAACTGCAACAGACATGTTCAGCTTTGACGATGC
CCATCCAAAAGTGACGCGGGATGAATATCGTTACAACGACATACCCGTTCGCTATCCGCAGCGGGTAGCGCAAGCACCTT
GCCTGCGTCCTTTTATCCAGTTGGAATTGATGGAAGCAAATTTGCTTGAGGCACCAGAGTTCAGGGATATTCATACACTG
GTGACGACACTTACTGGAAAAGCACCTGTCATTTCAGCCTTCCCCTGCGCCACAATTATCAGTACTCAGGCAGAAAAACT
GGTGGCTATGCTGCGCCGTACTGCAGCAGTTGCGCGTAATATTGAACGCCAGGATGACGCTTCGCTGGTACGTCATATCT
ACGACACATATTGTATCGTGCAGGCAGGCAATGTTGATATGTGTTTGCTTACTGATTTTTTTAAGCAAACCATTGAGCAA
GATATCCAGCGTTATGGCAATCAGTATCCGCAGTTTTGTGTTTCACCAAATGATGAGCTAAAAATGGGGCTGGACGAACT
TGAGCATAACCCGCTTTATAAGACACGCTACCAGCAATTTGTTGCCCCAATGGTATATGGTGCTCGCAAGGTATCGTGGG
ATGAAGCGTATGCTTCCTTTCGTCAAATGGCGTTAATAATTCTAGATACTTACTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT179656 WP_004902250.1 NZ_CP063931:c117698-117420 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT179656 NZ_CP084868:c914307-913912 [Klebsiella pneumoniae]
ATGACGATAAAAACACGCATTCAGTCACGACTGAAACGCTCAAAACGCTATGTATTCACTCGTGACGATTTCAAGGATAT
TGCAGGCTATGATCAGATTGGCAGGGTATTGCGCGACCTTCTCAAAGAGGGGCAACTACTGAAGGTGGGCTACGGTGTGT
ACACCAAAGCGCGTCGGAACAGCATTACTGGAAAAGTCATGCCCGCAGCACCAAGGGGATCTTCGGCAGTGATCGTTGAA
ACGCTGGAGCGCCTGAAAGTACGTTACCGCTATGAAGGAGCTACACAGACATATAATAGTGGCGAATCCACGCAAATCCC
CGTGTCACCGGAGATCAAAACATCTCCGCGCTTTAAACGCGCGTTGTCTGTGGGGAACAGCAAACTAAATGGATAA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3