Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 82133..83043 Replicon plasmid pM186-2
Accession NZ_CP063931
Organism Klebsiella pneumoniae strain M186-2

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag ISA67_RS25565 Protein ID WP_004026354.1
Coordinates 82133..82603 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag ISA67_RS25570 Protein ID WP_004026357.1
Coordinates 82600..83043 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA67_RS25545 77244..78086 + 843 WP_053065109.1 hypothetical protein -
ISA67_RS25555 79642..79890 + 249 Protein_58 transposase -
ISA67_RS25560 80953..81650 - 698 Protein_59 IS1 family transposase -
ISA67_RS25565 82133..82603 - 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
ISA67_RS25570 82600..83043 - 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
ISA67_RS25575 83144..83647 - 504 Protein_62 DUF4113 domain-containing protein -
ISA67_RS25580 83745..83966 - 222 WP_004213643.1 DUF2188 domain-containing protein -
ISA67_RS25585 84111..84266 - 156 WP_004213642.1 hypothetical protein -
ISA67_RS25590 84341..86467 - 2127 WP_004213640.1 TonB-dependent receptor family protein -
ISA67_RS25595 86554..87507 - 954 Protein_66 fec operon regulator FecR -
ISA67_RS25600 87504..88025 - 522 WP_004213635.1 sigma-70 family RNA polymerase sigma factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - fyuA / ybtE / ybtT / ybtU / irp1 / irp2 / irp2 / ybtA / ybtP / ybtQ / ybtX / ybtS / iroB / iroC / iroD / iroN / rmpA 1..191041 191041


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T179655 WP_004026354.1 NZ_CP063931:c82603-82133 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T179655 NZ_CP084868:851477-851761 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT179655 WP_004026357.1 NZ_CP063931:c83043-82600 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT179655 NZ_CP084868:851245-851487 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References