Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 6128..6932 Replicon plasmid pM186-2
Accession NZ_CP063931
Organism Klebsiella pneumoniae strain M186-2

Toxin (Protein)


Gene name KacT Uniprot ID -
Locus tag ISA67_RS25300 Protein ID WP_072198182.1
Coordinates 6128..6655 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID -
Locus tag ISA67_RS25305 Protein ID WP_048264996.1
Coordinates 6663..6932 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA67_RS25275 1768..2949 + 1182 WP_023345579.1 MFS transporter -
ISA67_RS25280 3068..3676 - 609 WP_023345578.1 short chain dehydrogenase -
ISA67_RS25285 3774..4676 + 903 WP_048265000.1 LysR family transcriptional regulator -
ISA67_RS25290 4988..5685 - 698 Protein_5 IS1 family transposase -
ISA67_RS25295 5736..6044 - 309 WP_064167953.1 hypothetical protein -
ISA67_RS25300 6128..6655 - 528 WP_072198182.1 GNAT family N-acetyltransferase Toxin
ISA67_RS25305 6663..6932 - 270 WP_048264996.1 DUF1778 domain-containing protein Antitoxin
ISA67_RS25310 7860..9035 + 1176 WP_077258344.1 SAVED domain-containing protein -
ISA67_RS25315 9032..10279 + 1248 WP_048264960.1 nucleotidyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - fyuA / ybtE / ybtT / ybtU / irp1 / irp2 / irp2 / ybtA / ybtP / ybtQ / ybtX / ybtS / iroB / iroC / iroD / iroN / rmpA 1..191041 191041
- flank IS/Tn - - 4988..5491 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(54-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 20078.04 Da        Isoelectric Point: 5.7389

>T179654 WP_072198182.1 NZ_CP063931:c6655-6128 [Klebsiella pneumoniae]
VMQELAIEMINDEFDYDIGEFDCGEETLNTFLKDHLKRQHDGQILKGYVLVTKEQKPKLLGYYTLSGSCFERKMLPSKTQ
QRKIPYQNAPSITLGRLAIDKSIQGQGWGELLVTHAMNVVWDASKAVGIYGLFVEALNDKAKRFYTRLDFIQLVGENSNL
LFYPTKSIEKIFTEE

Download         Length: 528 bp

>T179654 NZ_CP084868:693077-693994 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10148.65 Da        Isoelectric Point: 10.1733

>AT179654 WP_048264996.1 NZ_CP063931:c6932-6663 [Klebsiella pneumoniae]
MSALRKQRIDLRLTDDDKSLIEEAAAMSNQTITQFVVASASERAAEVIEQHRRMVLNEESWNLVMDAIANPAAPNERLKR
AAKRLQKMR

Download         Length: 270 bp

>AT179654 NZ_CP084868:692313-693071 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTTGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References