Detailed information of TA system TA179644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 4373904..4374494
• Replicon: chromosome
• Accession: NZ_CP063930
• Organism: Klebsiella pneumoniae strain M186-2

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: ISA67_RS20930
• Protein ID: WP_032421545.1
• Coordinates: 4374162..4374494 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: ISA67_RS20925
• Protein ID: WP_096106567.1
• Coordinates: 4373904..4374161 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA67_RS20900	4369740..4369982	-	243	WP_022615591.1	hypothetical protein	-
ISA67_RS20905	4370249..4371996	+	1748	Protein_4074	hypothetical protein	-
ISA67_RS20910	4372075..4372623	+	549	WP_096106565.1	hypothetical protein	-
ISA67_RS20915	4372906..4373366	+	461	Protein_4076	hypothetical protein	-
ISA67_RS20920	4373363..4373569	+	207	WP_096106566.1	helix-turn-helix domain-containing protein	-
ISA67_RS20925	4373904..4374161	+	258	WP_096106567.1	antitoxin	Antitoxin
ISA67_RS20930	4374162..4374494	+	333	WP_032421545.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ISA67_RS20935	4374781..4375992	+	1212	WP_038807401.1	hypothetical protein	-
ISA67_RS20940	4375996..4376490	+	495	WP_000881071.1	hypothetical protein	-
ISA67_RS20945	4376490..4378475	+	1986	WP_040220518.1	DUF3732 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4372075..4421388	49313

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11917.79 Da
• Isoelectric Point: 10.0968

>T179644 WP_032421545.1 NZ_CP063930:4374162-4374494 [Klebsiella pneumoniae]
MDRGEIWLVSLDPTSGHEQSGKRPVLIVSKASFNKLTQLPVIVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T179644 NZ_CP084866:c2736906-2736436 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9189.65 Da
• Isoelectric Point: 4.7468

>AT179644 WP_096106567.1 NZ_CP063930:4373904-4374161 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLGLSTDSEVGMTIDNGCLVIEPQKRPHYSLEELLAQCDPHAEISEGGREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT179644 NZ_CP084866:c2737346-2736903 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA