Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2055760..2056276 Replicon chromosome
Accession NZ_CP063930
Organism Klebsiella pneumoniae strain M186-2

Toxin (Protein)


Gene name relE Uniprot ID R4YAY3
Locus tag ISA67_RS09745 Protein ID WP_004178374.1
Coordinates 2055760..2056044 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID R4Y888
Locus tag ISA67_RS09750 Protein ID WP_002886901.1
Coordinates 2056034..2056276 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA67_RS09720 2051156..2051464 - 309 WP_012737232.1 PTS sugar transporter subunit IIB -
ISA67_RS09725 2051549..2051722 + 174 WP_032414379.1 hypothetical protein -
ISA67_RS09730 2051725..2052468 + 744 WP_002886905.1 MurR/RpiR family transcriptional regulator -
ISA67_RS09735 2052825..2054963 + 2139 WP_004186701.1 anaerobic ribonucleoside-triphosphate reductase -
ISA67_RS09740 2055292..2055756 + 465 WP_002886903.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
ISA67_RS09745 2055760..2056044 - 285 WP_004178374.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ISA67_RS09750 2056034..2056276 - 243 WP_002886901.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
ISA67_RS09755 2056354..2058264 - 1911 WP_009486549.1 BglG family transcription antiterminator -
ISA67_RS09760 2058287..2059441 - 1155 WP_023287050.1 lactonase family protein -
ISA67_RS09765 2059508..2060248 - 741 WP_002886899.1 KDGP aldolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-80)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 11155.97 Da        Isoelectric Point: 10.3787

>T179639 WP_004178374.1 NZ_CP063930:c2056044-2055760 [Klebsiella pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESTRLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Download         Length: 285 bp

>T179639 NZ_CP084861:c81657-81175 [Klebsiella pneumoniae]
GTGGGATGTATAACTGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGGGAAACGGTACTCGA
TGAATGGTTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCACGAACTTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTCCTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGACGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTTTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTTATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9045.55 Da        Isoelectric Point: 6.3340

>AT179639 WP_002886901.1 NZ_CP063930:c2056276-2056034 [Klebsiella pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Download         Length: 243 bp

>AT179639 NZ_CP084861:c81911-81645 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTCATTCTTGAGATGGCCTGCAAGGCTGCGGAGAACGTGATCCTTGATCGTCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCTGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A6THG1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GLP0

References