Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1905383..1906193 Replicon chromosome
Accession NZ_CP063930
Organism Klebsiella pneumoniae strain M186-2

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag ISA67_RS09170 Protein ID WP_002887280.1
Coordinates 1905383..1905916 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag ISA67_RS09175 Protein ID WP_002887278.1
Coordinates 1905927..1906193 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA67_RS09165 1904214..1905335 + 1122 WP_009309849.1 cupin domain-containing protein -
ISA67_RS09170 1905383..1905916 - 534 WP_002887280.1 GNAT family N-acetyltransferase Toxin
ISA67_RS09175 1905927..1906193 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
ISA67_RS09180 1906296..1907729 - 1434 WP_023287082.1 Cu(+)/Ag(+) sensor histidine kinase -
ISA67_RS09185 1907719..1908402 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
ISA67_RS09190 1908575..1909960 + 1386 WP_032414381.1 efflux transporter outer membrane subunit -
ISA67_RS09195 1909978..1910322 + 345 WP_023287080.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T179638 WP_002887280.1 NZ_CP063930:c1905916-1905383 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T179638 NZ_CP084861:c13981-13676 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACTTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT179638 WP_002887278.1 NZ_CP063930:c1906193-1905927 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT179638 NZ_CP084861:c14201-13983 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGATTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References