Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 157725..158250 Replicon plasmid pS183-1.2
Accession NZ_CP063929
Organism Klebsiella pneumoniae strain S183-1

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag ISA68_RS27090 Protein ID WP_001159871.1
Coordinates 157725..158030 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag ISA68_RS27095 Protein ID WP_000813630.1
Coordinates 158032..158250 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA68_RS27075 153687..154853 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
ISA68_RS27080 155441..156196 - 756 WP_000852146.1 replication initiation protein RepE -
ISA68_RS27085 156918..157724 - 807 WP_000016970.1 site-specific integrase -
ISA68_RS27090 157725..158030 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
ISA68_RS27095 158032..158250 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ISA68_RS27100 158810..159040 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
ISA68_RS27105 159037..159453 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
ISA68_RS27110 159528..161093 + 1566 WP_001128474.1 AAA family ATPase -
ISA68_RS27115 161078..162100 + 1023 WP_000361402.1 helicase UvrD -
ISA68_RS27120 162354..163040 - 687 Protein_211 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / aac(3)-IId / erm(B) / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-55 / blaTEM-1B / fosA3 / rmtB / sitABCD / ARR-3 / catB3 iutA / iucD / iucC / iucB / iucA 1..190929 190929
- inside Integron - iutA / iucD / iucC / iucB / iucA 364..190928 190564
- flank IS/Tn - - 162354..162701 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T179635 WP_001159871.1 NZ_CP063929:c158030-157725 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T179635 NZ_CP084860:c4621660-4621376 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT179635 WP_000813630.1 NZ_CP063929:c158250-158032 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT179635 NZ_CP084860:c4621892-4621650 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References