Detailed information of TA system TA179617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 88781..89451
• Replicon: plasmid pS183-1.1
• Accession: NZ_CP063928
• Organism: Klebsiella pneumoniae strain S183-1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: ISA68_RS25410
• Protein ID: WP_004213072.1
• Coordinates: 88781..89224 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: ISA68_RS25415
• Protein ID: WP_004213073.1
• Coordinates: 89221..89451 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA68_RS25375	84192..84467	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
ISA68_RS25380	84530..85021	+	492	WP_011251327.1	DM13 domain-containing protein	-
ISA68_RS25385	85070..85990	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
ISA68_RS25390	86081..86487	+	407	Protein_88	GAF domain-containing protein	-
ISA68_RS25395	87002..87638	-	637	Protein_89	response regulator	-
ISA68_RS25400	88054..88347	+	294	Protein_90	transposase	-
ISA68_RS25405	88351..88767	+	417	Protein_91	restriction endonuclease	-
ISA68_RS25410	88781..89224	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
ISA68_RS25415	89221..89451	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ISA68_RS25420	90059..91192	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
ISA68_RS25425	91208..91501	+	294	WP_004213076.1	hypothetical protein	-
ISA68_RS25430	91491..91697	-	207	WP_004213077.1	hypothetical protein	-
ISA68_RS25435	92049..92339	+	291	WP_004213078.1	hypothetical protein	-
ISA68_RS25440	92329..93228	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iucA / iucB / iucC / iucD / iutA / rmpA / rmpA2 / rmpA / iroN / iroD / iroC / iroB	1..208376	208376

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T179617 WP_004213072.1 NZ_CP063928:c89224-88781 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T179617 NZ_CP084859:895046-895456 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT179617 WP_004213073.1 NZ_CP063928:c89451-89221 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT179617 NZ_CP084859:894799-895065 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620