Detailed information of TA system TA179604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3471755..3472494
• Replicon: chromosome
• Accession: NZ_CP063927
• Organism: Klebsiella pneumoniae strain S183-1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: ISA68_RS16725
• Protein ID: WP_021312536.1
• Coordinates: 3472009..3472494 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: ISA68_RS16720
• Protein ID: WP_003026799.1
• Coordinates: 3471755..3472021 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA68_RS16705	3467258..3469327	+	2070	WP_194443773.1	glycine--tRNA ligase subunit beta	-
ISA68_RS16710	3469624..3470993	+	1370	WP_159210594.1	IS3 family transposase	-
ISA68_RS16715	3471194..3471622	+	429	WP_004901287.1	GFA family protein	-
ISA68_RS16720	3471755..3472021	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
ISA68_RS16725	3472009..3472494	+	486	WP_021312536.1	GNAT family N-acetyltransferase	Toxin
ISA68_RS16730	3472838..3472990	+	153	WP_002922102.1	type I toxin-antitoxin system toxin HokA	-
ISA68_RS16735	3473292..3474911	+	1620	WP_087830480.1	ATP-binding cassette domain-containing protein	-
ISA68_RS16740	3475010..3475222	-	213	WP_000014594.1	RNA chaperone/antiterminator CspA	-
ISA68_RS16745	3475475..3475765	-	291	WP_002921931.1	HTH-type transcriptional regulator	-
ISA68_RS16750	3476011..3477366	-	1356	WP_004901270.1	aromatic acid/H+ symport family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3470361..3470993	632

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17608.39 Da
• Isoelectric Point: 10.3370

>T179604 WP_021312536.1 NZ_CP063927:3472009-3472494 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T179604 NZ_CP084856:808153-808563 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT179604 WP_003026799.1 NZ_CP063927:3471755-3472021 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT179604 NZ_CP084856:807906-808172 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66