Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3195292..3195917 Replicon chromosome
Accession NZ_CP063927
Organism Klebsiella pneumoniae strain S183-1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag ISA68_RS15390 Protein ID WP_002882817.1
Coordinates 3195292..3195675 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag ISA68_RS15395 Protein ID WP_004150355.1
Coordinates 3195675..3195917 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA68_RS15375 3192658..3193560 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
ISA68_RS15380 3193557..3194192 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
ISA68_RS15385 3194189..3195118 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
ISA68_RS15390 3195292..3195675 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
ISA68_RS15395 3195675..3195917 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
ISA68_RS15400 3196122..3197039 + 918 WP_012737090.1 alpha/beta hydrolase -
ISA68_RS15405 3197053..3197994 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
ISA68_RS15410 3198039..3198476 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
ISA68_RS15415 3198473..3199333 - 861 WP_004146232.1 virulence factor BrkB family protein -
ISA68_RS15420 3199327..3199926 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T179603 WP_002882817.1 NZ_CP063927:c3195675-3195292 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T179603 NZ_CP084856:324412-324780 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT179603 WP_004150355.1 NZ_CP063927:c3195917-3195675 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT179603 NZ_CP084856:324194-324415 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References