Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4055790..4056421 Replicon chromosome
Accession NZ_CP063926
Organism Klebsiella variicola strain S187-1

Toxin (Protein)


Gene name hicA Uniprot ID A0A2V3KJT3
Locus tag ISA69_RS19300 Protein ID WP_044650325.1
Coordinates 4055790..4056065 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R4YIG2
Locus tag ISA69_RS19305 Protein ID WP_004181489.1
Coordinates 4056062..4056421 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA69_RS19280 4051026..4051361 + 336 WP_002921203.1 universal stress protein UspB -
ISA69_RS19285 4051421..4052917 - 1497 WP_008806938.1 inorganic phosphate transporter PitA -
ISA69_RS19290 4053147..4054340 + 1194 WP_012967106.1 NAD(P)/FAD-dependent oxidoreductase -
ISA69_RS19295 4054380..4055393 - 1014 WP_022066424.1 magnesium transporter -
ISA69_RS19300 4055790..4056065 + 276 WP_044650325.1 type II toxin-antitoxin system HicA family toxin Toxin
ISA69_RS19305 4056062..4056421 + 360 WP_004181489.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ISA69_RS19310 4056449..4056850 - 402 WP_023323361.1 nickel-responsive transcriptional regulator NikR -
ISA69_RS19315 4056838..4057629 - 792 WP_008806945.1 nickel import ATP-binding protein NikE -
ISA69_RS19320 4057626..4058390 - 765 WP_008806946.1 nickel import ATP-binding protein NikD -
ISA69_RS19325 4058390..4059223 - 834 WP_012967107.1 nickel ABC transporter permease subunit NikC -
ISA69_RS19330 4059220..4060164 - 945 WP_016161924.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10279.91 Da        Isoelectric Point: 10.3165

>T179595 WP_044650325.1 NZ_CP063926:4055790-4056065 [Klebsiella variicola]
MEQQVLSLRNKQRHTLEQLFKIPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

>T179595 NZ_CP084852:c3958922-3958605 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13313.02 Da        Isoelectric Point: 4.4605

>AT179595 WP_004181489.1 NZ_CP063926:4056062-4056421 [Klebsiella variicola]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp

>AT179595 NZ_CP084852:c3958612-3958325 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V3KJT3

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M5Q1

References